Purification of bovine and porcine enterokinase by affinity chromatography with immobilized kidney bean enterokinase inhibitor

Purification of pepsins and cathepsin D

Purification of pepsins and cathepsin D by affinity chromatography on Sepharose 4B with an immobilized synthetic inhibitor

✍ Jan Pohl; Milan Zaoral; Antonín Jindra Jr.; Vladimír Kostka 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 759 KB

Val-D-Leu-Pro-Phe-Phe-Val-D-Leu, a specific inhibitor of aspartate proteinases of the pepsin type, was synthesized. Its bonding to activated 6-aminohexanoic acid-Sepharose 4B afforded an affinity support suitable for the purification of human, porcine, and chicken pepsin, human gastricsin, and bovin