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Purification of small peptides labeled with Bolton-Hunter reagent

✍ Scribed by Stephen M. Coutts; Dorothy M. Reid

Book ID
102626150
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
296 KB
Volume
91
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method utilizing high-voltage electrophoresis on paper is described whereby a pentapeptide (Asp-Ser-Asp-Pro-Arg) labeled with Bolton-Hunter reagent is separated from hydrolyzed reagent and unreacted peptide and is recovered from the electrophoretogram in high yield. The general applicability to other peptides is discussed.

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