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Purification of Recombinant Human Transcription Factor IIB by Immunoaffinity Chromatography

✍ Scribed by N.E. Thompson; R.R. Burgess

Book ID
115644854
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
788 KB
Volume
5
Category
Article
ISSN
1046-5928

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Nuclease produced by recombinant Escherichia coli was successfully purified by immunoafftnity chromatography using an antibody preselected for the purpose on the basis of an elution assay. The elution assay was also used to optimize elution conditions. One step recovery of nuclease from crude peripl