In This New Edition Of The Very Successful Protein Purification Protocols (1996), Paul Cutler Completely Updates The Existing Protocols To Reflect Recent Advances And Adds An Enormous New Array Of Proteomic Techniques For Protein Isolation And Analysis. These Cutting-edge Techniques Include Not Only
✦ LIBER ✦
Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography
✍ Scribed by Irena Voráčková; Šárka Suchanová; Pavel Ulbrich; William E. Diehl; Tomáš Ruml
- Book ID
- 116829333
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 562 KB
- Volume
- 79
- Category
- Article
- ISSN
- 1046-5928
No coin nor oath required. For personal study only.
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This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his(6)-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co(2+) with iminodiacetate (IDA) as the chelating ligand, beta-glucuronid