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Purification of protein A, an outer membrane component missing in Escherichia coli K-12 ompA mutants

โœ Scribed by Tuu-jyi Chai; John Foulds


Book ID
113125594
Publisher
Elsevier Science
Year
1977
Weight
341 KB
Volume
493
Category
Article
ISSN
0005-2795

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The gene ompA encodes a major outer membrane protein of Escherichia coli. Localized mutagenesis of the part of the gene corresponding to the 21-residue signal sequence and the first 45 residues of the protein resulted in alterations which caused cell lysis when expressed. DNA sequence analyses revea