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Purification of pre-miR-29 by arginine-affinity chromatography

✍ Scribed by Pereira, Patrícia; Sousa, Ângela; Queiroz, João; Correia, Ilídio; Figueiras, Ana; Sousa, Fani

Book ID
125456846
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
761 KB
Volume
951-952
Category
Article
ISSN
1873-376X

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Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column