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Purification of glucosylceramidase by affinity chromatography

✍ Scribed by Strasberg, P. M. ;Lowden, J. A. ;Mahuran, D.


Book ID
111897742
Publisher
Canadian Science Publishing
Year
1982
Weight
719 KB
Volume
60
Category
Article
ISSN
0008-4018

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Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column