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Purification of pancreatic islets using hydroxyethyl starch–Collins solution

✍ Scribed by T Kenmochi; T Asano; K Jingu; Y Matsui; M Maruyama; H Miyauchi; T Ochiai

Book ID: 117230247
Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 90 KB
Volume: 33
Category: Article
ISSN: 0041-1345
DOI: 10.1016/s0041-1345(00)02194-1

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## Abstract Density-gradient purification of human pancreatic islets from the collagenase-digested pancreas relies on the exocrine tissue being denser than the islets. Cold storage of the pancreas before and after digestion causes cell swelling, which can decrease the density of pancreatic exocrine