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PURIFICATION OF NERVE GROWTH FACTOR ANTIBODIES BY AFFINITY CHROMATOGRAPHY

✍ Scribed by K. Stoeckel; C. Gagnon; G. Guroff; H. Thoenen


Book ID
111169491
Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
415 KB
Volume
26
Category
Article
ISSN
0022-3042

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Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column