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Purification of NADPH-linked α,β-ketoalkene double bond reductase from rat liver

✍ Scribed by Shigeyuki Kitamura; Kiyoshi Tatsumi

Book ID
115708376
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
650 KB
Volume
282
Category
Article
ISSN
0003-9861

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NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimm