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Purification of mouse pepsinogens by pepstatin-affinity chromatography

โœ Scribed by Hiroyasu Esumi; Shigeaki Sato; Takashi Sugimura; Chie Furihata


Book ID
115908271
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
317 KB
Volume
86
Category
Article
ISSN
0014-5793

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Purification of poliovirus by affinity c
โœ Fred Brown; Bryan O. Underwood; Karl H. Fantes ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 240 KB

Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column