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Purification of Mitochondria by Sucrose Step Density Gradient Centrifugation

โœ Scribed by Clayton, D. A.; Shadel, G. S.

Book ID
126544395
Publisher
Cold Spring Harbor Laboratory Press
Year
2014
Tongue
English
Weight
172 KB
Volume
2014
Category
Article
ISSN
1940-3402

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## Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory