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Purification of isoleucyl transfer ribonucleic acid synthetase by affinity chromatography on blue dextran—Sepharose

✍ Scribed by John G. Moe; Dennis Piszkiewicz


Book ID
115906910
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
341 KB
Volume
72
Category
Article
ISSN
0014-5793

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A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography