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Purification of human lysozyme from milk and pancreatic juice

✍ Scribed by Chi-Sun Wang; Hans-Ulrich Kloer

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
343 KB
Volume
139
Category
Article
ISSN
0003-2697

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✦ Synopsis

Human milk lysozyme was purified by heparin-Sepharose affinity chromatography and Sepharose 4B gel-permeation chromatography. This procedure was also found applicable to the purification of human pancreatic juice lysozyme. Double-diffusion analyses indicated that human milk lysozyme was immunochemically identical to human saliva and human pancreatic juice lysozyme. Based on the identity of the N-terminal 10-amino-acid-residue sequence analyzed, it was suggested that human milk lysozyme and human pancreatic juice lysozyme are identical molecular entities.

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