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Purification of human liver glycoprotein sialyltransferase by affinity chromatography

โœ Scribed by Jack A. Alhadeff; Robert T. Holzinger


Book ID
118387398
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
260 KB
Volume
6
Category
Article
ISSN
0165-022X

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Purification of pigeon liver malic enzym
โœ Jaw-Tang Chang; Gu-Gang Chang ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 336 KB

A simple and rapid method for the purification of malic enzyme (EC 1.1.1.40) from pigeon liver is described. Malic enzyme in the crude tissue extract was partially purified by heat treatment, ammonium sulfate fractionation, and DEAE-cellulose chromatography. Final purification was achieved by affini