Purification of human antibodies from transgenic corn using aqueous two-phase systems
β Scribed by J.-W. Lee; D. Forciniti
- Publisher
- American Institute of Chemical Engineers
- Year
- 2009
- Tongue
- English
- Weight
- 313 KB
- Volume
- 26
- Category
- Article
- ISSN
- 8756-7938
- DOI
- 10.1002/btpr.287
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π SIMILAR VOLUMES
Aqueous two-phase systems containing two polymers are a mild separation method for biomolecules due to the high concentration of water (75-90% ) in the phases. The phase systems have been further developed by introduction of thermoseparating polymers which make recycling of phase components possible
An effective system has been developed for purification of apolipoprotein A-1 from Escherichia coli fermentation solution and human plasma using aqueous two-phase extraction and thermal-phase separation. The system included non-ionic surfactants (Triton or Tween) and as top phase-forming polymer a r