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Purification of His-Tagged Proteins by Immobilized Chelate Affinity Chromatography: The Benefits from the Use of Organic Solvent

✍ Scribed by Kees L.M.C. Franken; Hoebert S. Hiemstra; Krista E. van Meijgaarden; Yanri Subronto; J. den Hartigh; Tom H.M. Ottenhoff; Jan W. Drijfhout


Book ID
115645582
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
68 KB
Volume
18
Category
Article
ISSN
1046-5928

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TEM-B-lactamase was purified by immobilized metal affinity chromatography from E. coil cell extracts. It was hypothesized that this protein could be purified in one step due to the abundence of metal binding residues. A pH gradient eluted the cell extract proteins. EDTA treatment released TEM-Blacta