Purification of glycollate oxidase from greening cucumber cotyledons

✍ Scribed by W. Behrends; U. Rausch; H. -G. Löffler; H. Kindl

• Book ID: 104753241
• Publisher: Springer-Verlag
• Year: 1982
• Tongue: English
• Weight: 848 KB
• Volume: 156
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00392782

✦ Synopsis

Glycollate oxidase (glycollate:oxygen oxidoreductase, EC 1.1.3.1) was purified to apparent homogeneity from crude extracts of greening cucumber cotyledons (Cucumis sativus). Molecular sieving and chromatofocusing resulted in 700-fold purification and specific activity of I gkat ragprotein. The enzyme exhibited a Mr of 180,000, or 700,000, respectively, and is a tetramer or 16-mer made of identical subunits of Mr 43,000. Monospecific antibodies were raised against the homogeneous protein.