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Purification of glutamine synthetase by adenosine-affinity chromatography

✍ Scribed by Mark Dowton; Ivan R. Kennedy

Book ID
107785531
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
312 KB
Volume
664
Category
Article
ISSN
1873-3778

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Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column