A new method for the purification of human erythrocyte phosphoglyceratekinase involving affinity chromatography on dye-ligand media (Red A), in the presence of 3-phosphoglycerate and ATP, is described. The method is rapid and technically simple. The purity of the enzyme was verified by electrophores
β¦ LIBER β¦
Preparative purification of adenosine deaminase from human erythrocytes by affinity chromatography
β Scribed by Josep M. Aran; Enric I. Canela; Rafael Franco
- Book ID
- 108449640
- Publisher
- Elsevier Science
- Year
- 1990
- Weight
- 723 KB
- Volume
- 532
- Category
- Article
- ISSN
- 0378-4347
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## Abstract A new group of specificβaffinity beads with metal chelates as ligands and synthetic polymer beads as support matrices was prepared and tested. This study focused on developing metalβchelated poly(hydroxyethyl methacrylate) (PHEMA) gel beads in a spherical form (100β150 ΞΌm in diameter) f