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Purification of free eukaryotic initiation factors eIF-4A and eIF-4D on cibacron blue F3G-A

✍ Scribed by Cor Van Der Mast; Harry O. Voorma

Book ID: 115747470
Publisher: Elsevier Science
Year: 1980
Weight: 993 KB
Volume: 607
Category: Article
ISSN: 0005-2787
DOI: 10.1016/0005-2787(80)90161-6

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## Abstract **Background:** The recruitment of mRNA for translation involves the assembly at the 5′cap of a complex of three initiation factors: the cap binding protein eIF4E, the ATP‐dependent RNA helicase eIF4A and the scaffold protein eIF4G. eIF4G mediates the binding of this mRNA–protein comple