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[Methods in Enzymology] Translation Initiation: Reconstituted Systems and Biophysical Methods Volume 430 || Expression and Purification of Recombinant Wheat Translation Initiation Factors eIF1, eIF1A, eIF4A, eIF4B, eIF4F, eIF(iso)4F, and eIF5

✍ Scribed by Mayberry, Laura K.

Book ID: 120029805
Publisher: Elsevier
Year: 2007
Tongue: English
Weight: 163 KB
Category: Article
ISBN: 0123739691
ISSN: 0076-6879
DOI: 10.1016/S0076-6879(07)30015-3

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## Abstract **Background:** The recruitment of mRNA for translation involves the assembly at the 5′cap of a complex of three initiation factors: the cap binding protein eIF4E, the ATP‐dependent RNA helicase eIF4A and the scaffold protein eIF4G. eIF4G mediates the binding of this mRNA–protein comple