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Purification of coenzyme a by affinity chromatography

โœ Scribed by Matuo, Yuhsi; Tosa, Tetsuya; Chibata, Ichiro


Book ID
122586153
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
517 KB
Volume
338
Category
Article
ISSN
0304-4165

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Purification of poliovirus by affinity c
โœ Fred Brown; Bryan O. Underwood; Karl H. Fantes ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 240 KB

Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column