Purification of Clostridium perfringens enterotoxins by high performance liquid chromatography
โ Scribed by Takashi Uemura; Yumiko Aoi; Yasuhiko Horiguchi; Akira Wadano; Naoki Goshima; Genji Sakaguchi
- Book ID
- 109312926
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 375 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A simple two-step procedure for obtaining highly purifed human tibrinopeptides is described. This technique utilizes batch adsorption to a Clll silica-coated matrix packed in a disposable cartridge. This step serves both to concentrate and desalt fibrinopeptides released into the supernatant during
Adenovirus (Type 2)-infected HeLa cells were sonicated and treated with 1,1,2-trichlorotrifluoroethane. The water-soluble extract was ultracentrifuged and the supernatant, containing the dissociated proteins, was subjected to anion-exchange high-performance liquid chromatography on a Mono Q column i