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Purification of Calf Prochymosin (Prorennin) Synthesized in Escherichia Coli

✍ Scribed by Marston, Fiona A. O. ;Lowe, Peter A. ;Doel, Michael T. ;Schoemaker, Joyce M. ;White, Susan ;Angal, Sarojani

Book ID
121412126
Publisher
Nature Publishing Group
Year
1984
Tongue
English
Weight
587 KB
Volume
2
Category
Article
ISSN
1087-0156

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The 1400 base pair repeat produced by digestion of calf satellite I DNA (phi = 1.714 g/cm3) with EcoRI, was cloned in E. coli. The hybrid plasmid (pGM 214) which contains the ColE1-Ap vector (pSF 2124) and the 1400 base pair fragment replicates stably in E. coli and can be amplified by chloramphenic