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Purification of Caenorhabditis elegans DNA topoisomerase I

โœ Scribed by Sang Mee Park; Hyeon-Sook Koo

Book ID
113263248
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
750 KB
Volume
1219
Category
Article
ISSN
0167-4781

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We have purified a topoisomerase activity from broccoli (Brassica oleracea var. italica) to near homogeneity. The enzyme is an 80 kDa monomer as judged by gel filtration chromatography and SDS gel electrophoresis, though it may represent a proteolytic fragment of a larger protein. The enzyme is capa