Purification and properties of DNA topoi
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Joseph J. Kieber; Mary F. Lopez; Alain F. Tissier; Ethan Signer
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Article
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1992
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Springer
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English
โ 570 KB
We have purified a topoisomerase activity from broccoli (Brassica oleracea var. italica) to near homogeneity. The enzyme is an 80 kDa monomer as judged by gel filtration chromatography and SDS gel electrophoresis, though it may represent a proteolytic fragment of a larger protein. The enzyme is capa