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Purification of bromelain using immobilized metal affinity membranes

✍ Scribed by Huali Nie; Shubai Li; Yuting Zhou; Tianxiang Chen; Zhiyan He; Sainan Su; Haitao Zhang; Yong Xue; Limin Zhu


Book ID
118473613
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
66 KB
Volume
136
Category
Article
ISSN
0168-1656

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An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody