Purification of bioproducts by free-flow zone electrophoresis: Choice of processing parameters
✍ Scribed by Dr. Hélène Roux-De Balmann; Rose-Marie Cerro; Victor Sanchez
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 1015 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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## Free flow electrophoresis for the purification of proteins: I. Zone electrophoresis and isotachophoresis The principles and some applications of free flow zone electrophoresis and isotachophoresis are described. The influence of (i) carrier electrolyte conductivity on the migration velocity and
Continuous, single-step, state-of-the-art preparative separations of enzymes from microorganism crude extracts by free-flow zone electrophoresis are presented. In the first example, the enzymes formate dehydrogenase, formaldehyde dehydrogenase, and methanol oxidase were continuously separated from C