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Purification of antithrombin III by affinity chromatography

✍ Scribed by Maggie Miller-Andersson; Håkan Borg; Lars-Olov Andersson


Book ID
119032977
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
867 KB
Volume
5
Category
Article
ISSN
0049-3848

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Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column