Purification of antigens of mammary tumor of the mouse by gel filtration
β Scribed by Calbert A. Laing; Knoxley M. Japal
- Book ID
- 113382567
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 450 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0885-4505
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## Abstract A radioimmunoassay for the antigens of the mouse mammary tumour virus has been developed. ^125^Iodineβlabelling of intact virus [derived from mammary tumours of (C3H Γ 020) F1 mice] followed by ether extraction and separation of the ether and water layers resulted in a separation of the
Antigens present in mouse mammary tumor M M 2 reacting with tumor-specific antibodies were extracted with 0.2 % deoxycholate. After the removal of deoxycholate by dialysis, most antigens were precipitated by centrifugation at 105,000 A g for 60 min. Hyperimmunization of C3HIHe mice with these antige