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Purification of Alpha Feto Protein from Human Cord Blood

✍ Scribed by Chaturvedi, R.; Agarkar, V.; Sharma, G. L.; Sarma, P. U.


Book ID
126757157
Publisher
Taylor and Francis Group
Year
1998
Tongue
English
Weight
460 KB
Volume
28
Category
Article
ISSN
1082-6068

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The AFP from human cord blood was isolated by means of affinity chromatography with the use of antibodies as ligands and by gel filtration. The preliminary purification was achieved by affinity chromatography on CNBr-Sepharose 4B coupled with anti AFP-antibody. Further purification was obtained by t