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Purification of alpha-fetoprotein from human cord serum with demonstration of its antiestrogenic activity

โœ Scribed by S.H.George Allen; James A. Bennett; Gerald J. Mizejewski; Thomas T. Andersen; Suellen Ferraris; Herbert I. Jacobson


Book ID
113269041
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
789 KB
Volume
1202
Category
Article
ISSN
0167-4838

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Human alpha-fetoprotein /AFP/ I. Isolati
โœ Maria Czokaล‚o ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 489 KB

The AFP from human cord blood was isolated by means of affinity chromatography with the use of antibodies as ligands and by gel filtration. The preliminary purification was achieved by affinity chromatography on CNBr-Sepharose 4B coupled with anti AFP-antibody. Further purification was obtained by t