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Purification of alcohol dehydrogenase from Bacillus stearothermophilus by affinity chromatography

✍ Scribed by M.J. Runswick; J.I. Harris

Book ID
115908877
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
185 KB
Volume
92
Category
Article
ISSN
0014-5793

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Gram quantities of homogeneous glycerokinase have been prepared from the thermophilic bacterium, Bacillus stearothermophilus, using three major steps: precipitation of debris at pH 5.1, ion-exchange chromatography on DEAE-Sephadex, and affinity chromatography on Procion Blue MX-3G-Sepharose. This me