✦ LIBER ✦

Purification of a recombinant protein produced in a baculovirus expression system by immobilized metal affinity chromatography

✍ Scribed by Min-Ying Wang; William E. Bentley; Vikram Vakharia

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 745 KB
Volume: 43
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260430502

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Suitability of immobilized metal affinit

Suitability of immobilized metal affinity chromatography for protein purification from canola

✍ Chen-Ming Zhang; Susan A. Reslewic; Charles E. Glatz 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 212 KB 👁 2 views

This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his(6)-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co(2+) with iminodiacetate (IDA) as the chelating ligand, beta-glucuronid

Purification and recognition of recombin

Purification and recognition of recombinant mouse P2X1 receptors expressed in a baculovirus system

✍ Liping Chen; James P. Hardwick; Peter McPhie; Michail V. Sitkovsky; Kenneth A. J 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 292 KB 👁 2 views

The effect of dissolved oxygen (DO) conc

The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell–baculovirus expression system

✍ Fuming Zhang; Mark A. Saarinen; Laura J. Itle; Sybil C. Lang; David W. Murhammer 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 141 KB 👁 2 views

Self-assembly of the infectious bursal d

Self-assembly of the infectious bursal disease virus capsid protein, rVP2, expressed in insect cells and purification of immunogenic chimeric rVP2H particles by immobilized metal-ion affinity chromatography

✍ Min-Ying Wang; Yung-Yan Kuo; Meng-Shiou Lee; Shyue-Ru Doong; Ji-Yi Ho; Long-Huw 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 2 views

A gene encoding a structural protein (VP2) of a local strain (P3009) of infectious bursal disease virus (IBDV) was cloned and expressed using the baculovirus expression system to develop a subunit vaccine against IBDV infection in Taiwan. The expressed rVP2 proteins formed particles of approximately

Tuning the expression level of recombina

Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system

✍ Jin-Ho Ahn; Taek-Jin Kang; Dong-Myung Kim 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 2 views

## Abstract In this work, it was discovered that the stability of mRNA in a cell‐free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequ