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Purification of a putative Na+ /D-glucose cotransporter from pig kidney brush border membranes on a phlorizin affinity column

✍ Scribed by Tomas Kitlar; Alison I. Morrison; Rolf Kinne; Josef Deutscher

Book ID: 115921304
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 639 KB
Volume: 234
Category: Article
ISSN: 0014-5793
DOI: 10.1016/0014-5793(88)81315-2

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In the presence of an NaSCN gradient phlorizin binds with a high affinity (Kd 4.7 pM) t o vesicles derived from brush border membranes of intestinal cells of rabbits. The value for Kd corresponds closely t o that of Ki determined from phlorizin inhibition of sugar transport. The apparent affinity fo