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N-ethylmaleimide labeling of a phlorizin-sensitive d-glucose binding site of brush border membrane from the rat kidney

✍ Scribed by L. Thomas; R. Kinne; P.P. Frohnert

Book ID: 115721625
Publisher: Elsevier Science
Year: 1972
Tongue: English
Weight: 567 KB
Volume: 290
Category: Article
ISSN: 0005-2736
DOI: 10.1016/0005-2736(72)90057-0

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In the presence of an NaSCN gradient phlorizin binds with a high affinity (Kd 4.7 pM) t o vesicles derived from brush border membranes of intestinal cells of rabbits. The value for Kd corresponds closely t o that of Ki determined from phlorizin inhibition of sugar transport. The apparent affinity fo