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Purification of a lipoxygenase from ungerminated barley Characterization and product formation

✍ Scribed by Peter G.M. van Aarle; Martina M.J. de Barse; Gerrit A. Veldink; Johannes F.G. Vliegenthart

Book ID
115924551
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
604 KB
Volume
280
Category
Article
ISSN
0014-5793

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Nitrite reductase (NiR) isolated from barley (Hordeum vulgare L.) roots was stabilized in a buffer solution containing a sulfhydryl-reducing reagent and glycerol. The enzyme was purified 340fold by ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50, Sephadex G-200 and DEAE-cellu