Purification, by high-performance liquid chromatography, and characterization, by high-field 1H-n.m.r. spectroscopy, of two fucose-containing pentasaccharides of goat's milk

✍ Scribed by Prasoon Chaturvedi; Chandra B. Sharma

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 681 KB
• Volume: 203
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(90)80048-8

✦ Synopsis

Two fucose-containing pentasaccharides were isolated fromgoat's milk using a Bio-Gel P-4column, followed by reverse-phase C-18 high-performance liquid chromatography.

The structures of the pentasaccharides as characterized by high-field 'H-n.m.r. spectroscopy and enzymatic digestion were found to be @Galp-( 1 -+4)-p-D-GlcpNAc-( 1+6)-B-D-Galp-( I -~4)-D-Gkp a-L-Fucp-( 1+ 3) and j?-D-Galp-( 1 +3)-/3-D-GlcpNAc-( l-+6)-P-D-Galp-( 1 -+D-Gkp