Characterization of metabolites in plant extracts of Ancistrocladus likoko by high-performance liquid chromatography coupled on-line with 1H NMR spectroscopy

✍ Scribed by Gerhard Bringmann; Markus Rückert; Wael Saeb; Virima Mudogo

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 117 KB
• Volume: 37
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/(sici)1097-458x(199902)37:2<98::aid-mrc428>3.0.co;2-i

✦ Synopsis

One-and two-dimensional stop-Ñow HPLCÈNMR experiments were applied to investigate a root extract of Ancistrocladus likoko (Ancistrocladaceae). Three new naphthylisoquinoline alkaloids with a 5,8@ coupling pattern were found. The constitutions and relative conÐgurations were elucidated by application of H,H-TOCSY and ROESY experiments carried out in the stop-Ñow mode. This example demonstrates the potential of HPLC-NMR as an efficient coupling technique for plant screening studies in phytochemical analysis.