Purification and Structural Analysis of the Hepatitis B Virus PreS1 Expressed from Escherichia coli
โ Scribed by Cheol-Young Maeng; Mee Sook Oh; Il Hyun Park; Hyo Jeong Hong
- Book ID
- 115587720
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 141 KB
- Volume
- 282
- Category
- Article
- ISSN
- 0006-291X
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The complete (encoding 119 aminon acids, aa) or partial (encoding the N-terminal 90 aa) preS1 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathione-S-transferase (GST) gene and expressed at 37 ยฐC under the control of the inducible tac promoter in E. coli. The results showed t
## Abstract The infectious particles of hepatitis B virus (HBV) contain 3 related surface antigens, i.e., small, medium, and large, all of which are encoded by one large open reading frame with multiple initiation codons. The large surface antigen (LโAg) contains preS1, preS2, and S regions while b