Expression and purification of the synthetic preS1 gene of Hepatitis B Virus with preferred Escherichia coli codon preference
β Scribed by Bingjun Qian; Huifeng Shen; Jingjing Xiong; Li Chen; Lu Zhang; Junwei Jia; Yuan Wang; Zhuchuan Zhang; Zheng Yuan; Kaiming Cao; Dabing Zhang
- Book ID
- 116828311
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 340 KB
- Volume
- 48
- Category
- Article
- ISSN
- 1046-5928
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The complete (encoding 119 aminon acids, aa) or partial (encoding the N-terminal 90 aa) preS1 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathione-S-transferase (GST) gene and expressed at 37 Β°C under the control of the inducible tac promoter in E. coli. The results showed t
## Abstract The infectious particles of hepatitis B virus (HBV) contain 3 related surface antigens, i.e., small, medium, and large, all of which are encoded by one large open reading frame with multiple initiation codons. The large surface antigen (LβAg) contains preS1, preS2, and S regions while b