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Purification and some properties of an α-amylase glucoamylase fusion protein from Saccharomyces cerevisiae

✍ Scribed by Lidia M. P. de Moraes; SpartacoAstolfi Filho; Cirano J. Ulhoa


Book ID
110271097
Publisher
Springer
Year
1999
Tongue
English
Weight
110 KB
Volume
15
Category
Article
ISSN
1573-0972

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Purification and some properties of sn-g
✍ Joseph R. Merkel; Martin Straume; Susan A. Sajer; Robert L. Hopfer 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 810 KB

An NAD-dependent glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate:NAD+ oxidoreductase, EC 1.1.1.8) has been isolated and purified from Saccharomyces cerevisiae by affinity and exclusion chromatography. The enzyme was purified 5 1 OO-fold to a specific activity of 158. It has a molecular w