Purification and some properties of a carboxymethyl cellulase from Favolus arcularius
β Scribed by Shougo Enokibara; Nobuhiro Mori; Yutaka Kitamoto
- Book ID
- 113396821
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 289 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0922-338X
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π SIMILAR VOLUMES
A newly isolated Bacillus species, which grew optimally at 30Β°C and pH 10, produced a carboxymethylcellulase in a medium containing 10 g CM-cellulose/l. The enzyme, when partially purified by gel filtration, had a mass of about 29 kDa as determined by both SDS-PAGE and gel filtration chromatography.
The extracellular carboxymethyl cellulase (CMCase) was purified 17-fold from Micrococcus roseus, a symbiotic organism of higher termites. Purified CMCase had an M r of 45 kDa and was optimally active at pH 8.0 and 40Β°C. Carbohydrate was associated with it and cellobiose was a competitive inhibitor o