Purification and Reconstitution of the Glutamate Carrier GltT of the Thermophilic Bacterium Bacillus stearothermophilus †
✍ Scribed by Gaillard, Isabelle; Slotboom, Dirk-Jan; Knol, Jan; Lolkema, Juke S.; Konings, Wil N.
- Book ID
- 127393599
- Publisher
- American Chemical Society
- Year
- 1996
- Tongue
- English
- Weight
- 329 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0006-2960
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## Abstract A carrier protein mediatine alanine transport was purified from the membranes of the thermophilic bacterium PS3, by ion exchange chromatography in the presence of both Triton X‐100 and urea. The alanine carrier was recovered in the nonadsorbed fraction from either DEAE‐or CM‐cellulose