𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Solubilization and partial purification of alanine carrier from membranes of a thermophilic bacterium and its reconstitution into functional vesicles

✍ Scribed by Hajime Hirata; Nobuhito Sone; Masasuke Yoshida; Yasuo Kagawa

Book ID
118311005
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
375 KB
Volume
69
Category
Article
ISSN
0006-291X

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Isolation of the alanine carrier from th
Isolation of the alanine carrier from the membranes of a thermophilic bacterium and its reconstitution into vesicles capable of transport
✍ Hirata, Hajime ;Sone, Nobuhito ;Yoshida, Masasuke ;Kagawa, Yasuo 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 425 KB

## Abstract A carrier protein mediatine alanine transport was purified from the membranes of the thermophilic bacterium PS3, by ion exchange chromatography in the presence of both Triton X‐100 and urea. The alanine carrier was recovered in the nonadsorbed fraction from either DEAE‐or CM‐cellulose

Transport of nutrients by a thermophilic
Transport of nutrients by a thermophilic bacterium—reconstitution of vesicles from crystalline ATPase or solubilized alanine carrier
✍ Yasuo Kagawa 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 406 KB

A strain of aerobic thermophilic bacteria was selected in order to purify highly stable membrane proteins and to reconstitute proteoliposomes capable of transporting nutrients from them. These proteins responsible for the transport could be divided into (1) proteins which supply energy to the transp