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Purification and properties of thermostable lipase from a thermophilic Bacillus stearothermophilus MC 7

โœ Scribed by M Kambourova; N Kirilova; R Mandeva; A Derekova


Book ID
114395950
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
163 KB
Volume
22
Category
Article
ISSN
1381-1177

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10 min. The enzyme released fructose exo-wise from the non-reducing end of inulin (M r = 4,5000). The Michaelis constant, catalytic center activity, and specificity constant for inulin at 60 ยฐC and pH 5.0 were 80 mM (360 mg/mL), 460 s -1 , and 5.8 s -1 mM -1 , respectively. The ratio of specificity