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Purification and properties of the clotting enzyme from limulus lysate

✍ Scribed by James D. Sullivan Jr.; Stanley W. Watson


Book ID
118310212
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
698 KB
Volume
66
Category
Article
ISSN
0006-291X

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Purification and properties of milk-clot
✍ Lavu Krishna Rao; D. K. Mathur πŸ“‚ Article πŸ“… 1979 πŸ› John Wiley and Sons 🌐 English βš– 524 KB

## Abstract A milk‐clotting enzyme from __Bacillus subtilis__ K‐26 was purified by gel filtration and ion‐exchange chromatography resulting in a 24‐fold increase in specific activity with an 80% yield. Polyacrylamide gel electrophoresis and ultracentrifugel analysis revealed that the purified enzym