Purification and Properties of Human Erythrocyte Pyrimidine 5′-nucleotidase
✍ Scribed by J. D. Torrance, D. Whittaker and E. Beutler
- Book ID
- 123654461
- Publisher
- National Academy of Sciences
- Year
- 1977
- Tongue
- English
- Weight
- 903 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/67018
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📜 SIMILAR VOLUMES
A new case of a defect in red cell pyrimidine 5'-nucleotide (P5N) activity was found in a large family from Guadeloupe in the West Indies. The propositus presented a characteristic hemolytic anemia with red cell basophilic stippling, an increased GSH level, and a shift of the peak in absorbance of n
The mutant enzyme of a patient with hereditary pyrimidine 5'-nucleotidase deficiency was analyzed biochemically. Partially purified by DEAE-Sephadex and concentrated by ultrafiltration, the enzyme had a high Km for the substrate uridine monophosphate. Utilization of the substrate cytidine monophosph