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Purification and properties of glucose isomerase of Actinoplanes missouriensis

✍ Scribed by Cheng-Shung Gong; Li Fu Chen; George T. Tsao

Publisher: John Wiley and Sons
Year: 1980
Tongue: English
Weight: 494 KB
Volume: 22
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260220408

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✦ Synopsis

Abstract

Actinoplanes missouriensis produces an intracellular soluble glucose Isomerase. The soluble enzyme can be purified by a DEAE‐cellulose beads columm with a onestep salt elution. The purified enyzme exhibited a molecular weight of approximately 80,000 daltons, being composed of two identical subunits of about 42,000 daltons each. The K~m~ for glucose is 1.33__M__, the K~m~ for frucotse is 1.67__M__. The enzyme has an optimal pH of 7.0. The presence of the cobalt ion is not required to produce optimal activity of the enzyme if the proper amount of magnesium is present.

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